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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 9, No. 5, 2006, pp. 566-571
Bioline Code: ej06076
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 9, No. 5, 2006, pp. 566-571

 en Modification of the activity of an α-amylase from Bacillus licheniformis check for this species in other resources by several surfactants
Rodríguez, Vicente Bravo; Alameda, Encarnación Jurado; Gallegos, Juan Francisco Martínez; Requena, Antonia Reyes; López, Ana Isabel García; Cabral, Joaquim Manuel Sampaio; Fernandes, Pedro & da Fonseca, Luis Joaquim Pina

Abstract

The influence of different commercial surfactants on the enzymatic activity of a commercial α-amylase from Bacillus licheniformis check for this species in other resources (Termamyl 300 L) has been studied. As non-ionic surfactants, alkyl polyglycosides (Glucopon® 215, Glucopon® 600 and Glucopon® 650) were studied, as were fatty alcohol ethoxylates (Findet 1214N/23 and Findet 10/15), and nonyl phenol ethoxylate (Findet 9Q/21.5NF). Also, an anionic surfactant, linear alkyl benzene sulfonate (LAS) was assayed. In general, none of the non-ionic surfactants studied, except Findet 10/15, vary substantially the enzymatic activity. Findet 10/15 has the strongest hydrophobic character and reduces the enzymatic activity more significantly the greater its concentration. Regarding LAS, this surfactant significantly depressed enzymatic activity, presumably due to the electrostatic interactions caused by its anionic character.

Keywords
α-amylase, alkyl polyglycosides, enzymatic activity, fatty alcohol ethoxylates, linear alkyl benzene sulfonate, nonyl phenol ethoxylate.

 
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