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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 9, No. 3s1, 2006, pp. 181-186
Bioline Code: ej06083
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 9, No. 3s1, 2006, pp. 181-186

 en A new variety of Bacopa monnieri check for this species in other resources obtained by in vitro polyploidization
Escandón, Alejandro Salvio; Hagiwara, Juan Carlos & Alderete, Liliana Marisol

Abstract

The commercial value of Bacopa monnieri check for this species in other resources , a widespread herbaceous plant in Argentina , can be substantially improved increasing its flower size by chromosome doubling with colchicine. MS supplemented with 0.25 mg/L of 6-benzylaminopurine proved to be an appropriate medium for the in vitro multiplication of nodal segments of B. monnieri. Polyploidization was achieved submerging nodal segments during 24 or 48 hrs in colchicine solution 0.001 and 0.01% P/V, in 1% DMSO. Segments submerged in water and in DMSO 1% aqueous solution were used as controls. DNA contents from recovered plants was measured and characterized and their phenotype described. Two tetraploid plants originated from independent events were detected. These plants showed significant differences in size and colour both in leaves and flowers compared to untreated controls.

Keywords
colchicine, flow cytometry, ornamental plants, Scrophulariaceae, tetraploid.

 
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