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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 9, No. 3s1, 2006, pp. 187-194
Bioline Code: ej06084
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 9, No. 3s1, 2006, pp. 187-194

 en An alternative pathway for plant in vitro regeneration of chinaberry -tree Melia azedarach check for this species in other resources L. derived from the induction of somatic embryogenesis
Sharry, Sandra; Ponce, José Luis Cabrera; Estrella, Luis Herrera; Cano, Rosa María Rangel; Lede, Silvia & Abedini, Walter

Abstract

A highly efficient somatic embryogenesis system and subsequent plant regeneration of chinaberry ( Melia azedarach check for this species in other resources L.) was developed. Plants were regenerated from indirect somatic embryogenesis induction. Novel features of this improved protocol, include: a) Embryogenic callus induction with no addition of 2, 4-D in the culture media; b) Somatic embryos differentiation was achieved by using high concentration of cytokinins (BAP 10 mg/L) and adenine; c) 100% conversion of somatic embryos to plants was practically obtained and 100% of plants survived under greenhouse conditions; d) Addition of putrescine improved somatic embryos germination. The amount of somatic embryos produced by the pathway of indirect somatic embryogenesis was 447 per gram of fresh weight callus. Regenerated plants were phenotypically normal. The developed protocol established the potential to produce plantlets from cotyledon explants through somatic embryogenesis. It also presents itself as a highly efficient method for mass clonal propagation and conservation of Melia azedarach.

Keywords
callus induction, clonal forestry, embryos conversion, micropropagation, persian lilac, somatic embryos.

 
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