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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 9, No. 3s1, 2006, pp. 248-252
Bioline Code: ej06095
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 9, No. 3s1, 2006, pp. 248-252

 en Construction of a molecular identification profile of new varieties of Nierembergia linariaefolia check for this species in other resources by anchored microsatellites
de la Torre, Mariana C. Pérez & Escandón, Alejandro S.

Abstract

The objective of the present work was to establish the molecular identification profile for six new varieties of Nierembergia linariaefolia check for this species in other resources to incorporate the fingerprint, as complementary information to the standard registration data. Total DNA was extracted from young leaves following the protocol of the cetyltrimethylammonium bromide. Anchored microsatellites were used as molecular markers. The amplification reactions were carried out with seven primers. A total of 251 loci were detected, 98% of them were polymorphic. The average of polymorphic loci was 35 loci per primer and, 41 loci per genotype. Six out of the seven primers used discriminated all the individuals involved in the present study; consequently, it was possible to generate the molecular identification profile for the six new varieties. This result, supported together with our previous reports, indicates that the anchored microsatellites are a very useful technique for the fingerprints generation in N. linariaefolia.

Keywords
ISSR, ornamentals, varietal identification.

 
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