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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 10, No. 1, 2007, pp. 1-11
Bioline Code: ej07001
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 10, No. 1, 2007, pp. 1-11

 en Application of statistical experimental methods to optimize medium for exopolymer production by newly isolated Halobacterium sp. check for this species in other resources SM5
Lungmann, Patcharee; Choorit, Wanna & Prasertsan, Poonsuk


The study investigated on medium optimization for production of exopolymer by a newly isolated bacterium, Halobacterium sp. check for this species in other resources SM5, using the mixture design and response surface method. The mixture experiment was designed by setting five parameters at the lowest and highest of concentrations: pseudo components design containing 0.15-0.25 glucose, 0.15-0.25 yeast extract, 0.35-0.45 MgSO4·7H2O, 0.1-0.2 vitamin casamino acid and 0.02-0.06 KCl. The results of the mixture design revealed that the effect of nutrients or elements on the exopolymer produced by the strain SM5 were in the order of MgSO4·7H2O > yeast extract > vitamin casamino acid > KCl > glucose, respectively. The suitable medium recipe for enhancement the exopolymer production was 7.43 g/l glucose, 12.38 g/l yeast extract, 17.33 g/l MgSO4·7H2O, 9.9 g/l vitamin casamino acid and 2.48 g/l KCl. The exopolymer to be produced by the strain SM5 was 2.25 g/l, which was higher than that obtained in the original medium (1.3 times). The yield of exopolymer was 2.13 g/l to be obtained in medium containing 7.43 g/l glucose, 11.37 g/l yeast extract, 22.28 g/l MgSO4·7H2O, 7.44 g/l vitamin casamino acid and 0.99 g/l KCl which was predicted by response surface methodology. However, under an experiment, the yield of exopolymer was 2.08 ± 0.0020 g/l.

exopolymer, halophilic bacteria, medium optimization, mixture design, response surface method.

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