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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 10, No. 1, 2007, pp. 1-6
Bioline Code: ej07015
Full paper language: English
Document type: Short Communication
Document available free of charge

Electronic Journal of Biotechnology, Vol. 10, No. 1, 2007, pp. 1-6

 en Practical use of immobilized lysozyme for the remediation process of Escherichia coli check for this species in other resources in aqueous solution
Alves, Carlucio R.; Pimenta, Maria Gardenny R.; Vieira, Regine H. S. F.; Furtado, Roselayne F.; Guedes, Maria Izabel F.; Silva, Rui C. B. & Assis, Odilio B. G.

Abstract

The lysozyme enzyme was immobilized on vitreous surface (fragments with diameters of 0.3 and 1.0 mm ) for remediation of the microorganism Escherichia coli check for this species in other resources JM 109 into fresh water and saline solutions with 0.9% NaCl (w/v). Characterization of enzymatic film was carried out by infrared spectroscopy and atomic force microscopy techniques. Bactericide activity of the enzyme was evaluated by spectrophotometric analysis. It was verified that the enzymatic film was strongly coupled with the vitreous surface. The topographic analysis demonstrated that the deposited film was uniform and homogeneous. It was observed bactericide activity of film deposited on vitreous surface with 0.3 mm in fresh and saline solutions. This fact was not verified to vitreous fragments with 1.0 mm of diameter.

Keywords
Escherichia coli, lysozyme, self-assembly.

 
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