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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 10, No. 1, 2007, pp. 1-3
Bioline Code: ej07016
Full paper language: English
Document type: Note
Document available free of charge

Electronic Journal of Biotechnology, Vol. 10, No. 1, 2007, pp. 1-3

 en Ethanol mediated enhancement in bacterial transformation
Sharma, Arun Dev; Singh, Jaspreet & Gill, Prabhjot Kaur

Abstract

In molecular biology, transformation using E. coli as a host plays a key role in synthesizing gene libraries. The present study demonstrated a new ethanol-based method for transformation of plasmid DNA to E. coli. Ethanol at 10% concentration (v/v) showed best results. Further, as compared with traditional CaCl2 method, the transformation rate, using protocol outlined in this study, was very high, suggesting amenable for further applications.

Keywords
bacteria, ethanol, transformation.

 
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