Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
Vol. 10, No. 1, 2007, pp. 1-3
Bioline Code: ej07016
Full paper language: English
Document type: Note
Document available free of charge
Electronic Journal of Biotechnology, Vol. 10, No. 1, 2007, pp. 1-3
© Copyright 2007 - Pontificia Universidad Católica de Valparaíso -- Chile
Ethanol mediated enhancement in bacterial transformation|
Sharma, Arun Dev; Singh, Jaspreet & Gill, Prabhjot Kaur
In molecular biology, transformation using E. coli as a host plays a key role in synthesizing gene libraries. The present study demonstrated a new ethanol-based method for transformation of plasmid DNA to E. coli. Ethanol at 10% concentration (v/v) showed best results. Further, as compared with traditional CaCl2 method, the transformation rate, using protocol outlined in this study, was very high, suggesting amenable for further applications.
bacteria, ethanol, transformation.
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