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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 10, No. 2, 2007, pp. 322 - 327
Bioline Code: ej07030
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 10, No. 2, 2007, pp. 322 - 327

 en SHORT COMMUNICATION - Functional complementation in Saccharomyces cerevisiae under control of the natural yeast promoter
Lavigne, Rob & Volckaert, Guido

Abstract

A novel approach for functional complementation of foreign genes in Saccharomyces cerevisiae is presented. This approach is based on the use of the widely available cognate gene plasmids (e.g. pRS416) of the European Functional Analysis Network (EUROFAN). The functional complementation of the human homolog of YOR159c (SME1 gene) shown here is the first demonstration of complementation using the original yeast promoter, theoretically offering a more natural regulation of protein expression.

Keywords
cognate gene plasmid, functional complementation, homolog, Saccharomyces cerevisiae, yeast.

 
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