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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 10, No. 3, 2007, pp. 436-443
Bioline Code: ej07042
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 10, No. 3, 2007, pp. 436-443

 en Molecular description and similarity relationships among native germplasm potatoes ( Solanum tuberosum ssp. tuberosum L. check for this species in other resources ) using morphological data and AFLP markers
Solis, Jaime Solano; Ulloa, Daniza Morales & Rodríguez, Leonardo Anabalón

Abstract

Chile is considered to be a sub-center of origin for the cultivated potato, with native and introduced genetic material coexisting in the country. Thus, the different varieties present in Chiloe Island are characterized by a rich diversity of forms, sizes, colours and phenological characteristics. In the present work, the level of polymorphism and the genetic relationship were studied by means of molecular markers using the amplified fragment length polymorphism (AFLP) technique and twenty-seven morphological characters. Twenty varieties of potatoes from the Chiloe Island were analyzed. The commercial variety Desirée and one specie from the Etuberosa series, Solanum fernandezianum, collected in the Juan Fernandez Island were included as controls. A similarity tree-diagram was made, based on all the AFLP bands generated in the range between 65 and 290 base pairs. With these tools, it was possible to identify molecular differences and similarities that might be associated with important morphological traits such as the predominant forms of the tuber, flower colour and resistance to disease.

Keywords
cluster analysis, molecular markers, Solanum tuberosum ssp. tuberosum

 
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