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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 10, No. 3, 2007, pp. 444-451
Bioline Code: ej07043
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 10, No. 3, 2007, pp. 444-451

 en Trametes versicolor check for this species in other resources growth and laccase induction with by-products of pulp and paper industry
Xavier, Ana Maria Rebelo Barreto; Tavares, Ana Paula Mora; Ferreira, Rita & Amado, Francisco

Abstract

The cultivation of Trametes versicolor for laccase production and cell growth were strongly dependent on experimental conditions namely physical and chemical parameters as well as nutrient availability and inducer stimulation. Biomass growth was compared for a rich medium and for a defined medium in two different temperatures. The best temperature was 28°C and the maximum specific growth rates were μmax = 0.083 h-1 for the rich medium and μmax = 0.043 h-1 for the defined medium. It was clearly shown that laccase production is not associated with cell growth, indicating that this ligninolytic enzyme must be produced in the defined medium by a secondary metabolism. In order to obtain laccase induction, addition of solid lignin, lignosulphonates, veratryl alcohol, xylidine and ethanol was tested at different concentrations. To optimise laccase activity, the combined effect of inducer addition and simultaneously glucose suppression was studied. The best result for laccase induction (1240 U/L) was obtained with solid lignin, a by-product of pulp and paper industry and the higher laccase activity attained (1583 U/L) was obtained with the combined effect of xylidine addition and glucose suppression.

Keywords
inducers, laccase, pulp and paper by-products, Trametes versicolor.

 
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