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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 10, No. 3, 2007, pp. 468-472
Bioline Code: ej07045
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 10, No. 3, 2007, pp. 468-472

 en TECHNICAL NOTE - A simple method for isolation of genomic DNA from fresh and dry leaves of Terminalia arjuna check for this species in other resources (Roxb.) Wight and Arnot
Deshmukh, Vishal P.; Thakare, Prashant V.; Chaudhari, Uddhav S. & Gawande, Prashant A.

Abstract

Current protocols for isolation of genomic DNA from Terminalia arjuna have their own limitations due to the presence of high content of gummy polysaccharides and polyphenols. DNA isolated by these protocols is contaminated with a yellowish, sticky and viscous matrix. In our modified DNA isolation method polysaccharides and polyphenols are removed prior to the precipitation of the DNA. Then the genomic DNA was precipitated using isopropanol. This protocol yielded a high molecular weight DNA isolated from fresh as well as dry leaves of T. arjuna, which was free from contamination and colour. Isolated DNA can be used for restriction digestion and PCR amplification. The main objective of the present protocol is to provide a simple method of isolation of DNA, using in house prepared reagents.

Keywords
Ayurveda, DNA extraction, medicinal plant, Terminalia species.

 
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