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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 10, No. 3, 2007, pp. 473 - 480
Bioline Code: ej07046
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 10, No. 3, 2007, pp. 473 - 480

 en TECHNICAL NOTE - Isolation of simple sequence repeats from groundnut
Wang, Chuan Tang; Yang, Xin Dao; Chen, Dian Xu; Yu, Shan Lin; Liu, Guang Zhen; Tang, Yue Yi & Xu, Jian Zhi

Abstract

SSRs have proved to be the most powerful tool for variety identification in groundnut of similar origin, and have much potential in genetic and breeding studies. To facilitate SSR discovery in groundnut, we proposed a highly simplified SSR isolation protocol based on multiple enzyme digestion/ligation, mixed biotin-labeled probes and streptavidin coated magnetic beads hybridization capture strategy. Of the 272 colonies randomly picked for sequencing, 119 were found to have unique SSR inserts.

Keywords
groundnut, isolation, simple sequence repeat.

 
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