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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 10, No. 4, 2007, pp. 582-591
Bioline Code: ej07057
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 10, No. 4, 2007, pp. 582-591

 en Micropropagation of Lilium ledebourii check for this species in other resources (Baker) Boiss as affected by plant growth regulator, sucrose concentration, harvesting season and cold treatments
Pejman Azadi & Morteza Khosh-Khui


A protocol for the micropropagation in different harvesting time of Lilium ledebourii check for this species in other resources (Baker) Boiss, an endangered rare species endemic to Iran has been developed. In vitro scale culture of this species, using bulbs from three harvesting seasons (spring, summer and winter), was attempted. Among the various treatments tested, the Murashige and Skoog (MS) medium supplemented with 0.1 mg l-1 naphthaleneacetic acid (NAA) + 0.1 mg l-1 benzyladenin (BA) and 6% sucrose in all harvesting seasons proved to be superior to others. The best results for fresh weight of bulblets, rooting parameters and the survival rate after transplantation to greenhouse were obtained from early winter-harvested bulbs. Summer-harvested bulbs had the highest number of bulblets per explant. The bulblets at the end of the culture period were given cold treatment at 4°C for 2-8 weeks at a 2-weeks interval and then transplanted to a potting mixture of sand, leaf mold and peat moss (1:1:1 v/v). The best emergence rate (90%) was achieved at 8 weeks cold treatment for winter harvested bulbs.

bulblet, cold treatment, conservation, harvesting season, Lilium ledebourii, tissue culture.

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