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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 10, No. 4, 2007, pp. 627-632
Bioline Code: ej07061
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 10, No. 4, 2007, pp. 627-632

 en Phage-resistance of Salmonella enterica check for this species in other resources serovar Enteritidis and pathogenesis in Caenorhabditis elegans check for this species in other resources is mediated by the lipopolysaccharide
Santander, Javier & Robeson, James

Abstract

Phage therapy has been used in the past as an alternative therapy against bacterial pathogens. However, phage-resistant bacterial strains can emerge. Some studies show that these phage-resistant strains are avirulent. In this study, we report that phage-resistant strains of Salmonella enterica check for this species in other resources serovar Enteritidis (hereafter S. Enteritidis) were avirulent in the Caenorhabditis elegans check for this species in other resources animal model. We isolated phage-resistant strains of S. Enteritidis ATCC 13076 by using three lytic phages (f2α;SE, f3α;SE and f18α;SE). In these mutants, we explored different virulence factors like lipopolysaccharide (LPS), virulence plasmid (Pla), motility and type I fimbriae, all of which may have effects on virulence and could furthermore be related to phage resistance. The phage-resistant strains of S. Enteritidis showed loss of O-Polysaccharide (O-PS) and auto-agglutination, present a rough phenotype and consequently they are avirulent in the C. elegans animal model. We speculate that the O-PS is necessary for phage attachment to the S. Enteritidis cell surface.

Keywords
animal model, bacteriophage prophylaxis, Caenorhabditis elegans, phage resistance, Salmonella.

 
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