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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 10, No. 4, 2007, pp. 633-637
Bioline Code: ej07062
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 10, No. 4, 2007, pp. 633-637

 en Short Communication - Methyl jasmonate induced overproduction of eleutherosides in somatic embryos of Eleutherococcus senticosus check for this species in other resources cultured in bioreactors
Shohael, Abdullah Mohammad; Murthy, Hosakatte Niranjana; Hahn, Eun-Joo & Paek, Kee-Yoeup

Abstract

This study was concentrated on the production of eleutherosides and chlorogenic acid in embryogenic suspension cultures of Eleutherococcus senticosus check for this species in other resources by exposing them to different concentrations (50-400 μM) of methyl jasmonate (MJ) during the culture period. In the bioreactor cultures, eleutheroside content increased significantly by elicitation of MJ, however, the fresh weight, dry weight and growth ratio of embryos was strongly inhibited by increasing MJ concentrations. The highest total eleutheroside (7.3 fold increment) and chlorogenic acid (3.9 fold increment) yield was obtained with 200 μM MJ treatment. There was 1.4, 3.4 and 14.9 fold increase in the eleutheroside B, E, and E1 production respectively with such elicitation treatment. These results suggest that MJ elicitation is beneficial for eleutheroside accumulation in the embryogenic cell suspension cultures.

Keywords
eleutherosides, elicitation, methyl jasmonate, secondary metabolite, siberian ginseng, suspension culture.

 
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