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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 11, No. 1, 2008
Bioline Code: ej08007
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 11, No. 1, 2008

 en Overexpression of the pineapple fruit bromelain gene (BAA) in transgenic Chinese cabbage ( Brassica rapa check for this species in other resources ) results in enhanced resistance to bacterial soft rot
Jung, Yu-Jin; Choi, Chang-Sun; Park, Jin-Heui; Kang, Hee-Wan; Choi, Je-Eul; Nou, Ill-Sup; Lee, Soon Youl & Kang, Kwon-Kyoo

Abstract

Bromelain is a crude protein extract obtained from pineapple stems, which comprises a variety of proteolytic enzymes. It exhibits potential therapeutic activities against trauma, inflammation, autoimmune diseases and malignant disorders. In this study, we cloned BAA1 (the gene encoding fruit bromelain) into a plant expression vector that was then used to transform Brassica rapa check for this species in other resources and overexpress BAA1 under the control of the cauliflower mosaic virus (CaMV) 35S promoter. We demonstrate that constitutive overexpression of BAA1 in B. rapa confers enhanced resistance to the soft rot pathogen Pectobacterium carotovorum ssp. carotovorum check for this species in other resources . These results suggest that it could be utilized for protecting plants from attack by bacterial pathogens.

Keywords
Brassica rapa, fruit bromelain, soft rot resistance, transgenic plant.

 
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