Bromelain is a crude protein extract obtained from pineapple stems, which comprises a variety of proteolytic enzymes. It exhibits potential therapeutic activities against trauma, inflammation, autoimmune diseases and malignant disorders. In this study, we cloned BAA1
(the gene encoding fruit bromelain) into a plant expression vector that was then used to transform Brassica rapa
and overexpress BAA1
under the control of the cauliflower mosaic virus (CaMV) 35S promoter. We demonstrate that constitutive overexpression of BAA1
in B. rapa
confers enhanced resistance to the soft rot pathogen Pectobacterium carotovorum
. These results suggest that it could be utilized for protecting plants from attack by bacterial pathogens.