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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 11, No. 1, 2008
Bioline Code: ej08013
Full paper language: English
Document type: Short Communication
Document available free of charge

Electronic Journal of Biotechnology, Vol. 11, No. 1, 2008

 en Agrobacterium check for this species in other resources -mediated transient transformation of Mexican prickly poppy ( Argemone mexicana check for this species in other resources L.)
Godoy-Hernández, Gregorio; Avilés-Berzunza, Elidé; Carrillo-Pech, Mildred & Vázquez-Flota, Felipe

Abstract

Shoot apex, leaf primordia, leaf sections and roots from Mexican prickly poppy seedlings, were inoculated with Agrobacterium tumefaciens check for this species in other resources harboring the binary vector pCAMBIA2301, which contained the β-glucuronidase (uid A) gene. Histochemical β-glucuronidase (GUS) assay in infected explants showed transient gus gene expression between 3 and 12 days after inoculation. To our knowledge, this is the first report of A. mexicana susceptibility to A. tumefaciens-mediated genetic transformation.

Keywords
Argemone mexicana, β-glucuronidase, benzylisoquinoline alkaloids, genetic transformation, neomycin phosphotransferase II.

 
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