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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 11, No. 2, 2008
Bioline Code: ej08025
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 11, No. 2, 2008

 en Induction of in vitro flowering in Capsicum frutescens check for this species in other resources under the influence of silver nitrate and cobalt chloride and pollen transformation
Sharma, A.; Kumar, V.; Giridhar, P. & Ravishankar, G.A.

Abstract

The influence of silver nitrate (AgNO3) and cobalt chloride (CoCl2) on shoot multiplication and in vitro flowering in Capsicum frutescens check for this species in other resources Mill. was investigated. Exogenous administration of AgNO3 and CoCl2 at a concentration of 30 µM resulted in the maximum tissue response in terms of shoot length and number of shoots after 45 days culturing on MS medium. Both silver nitrate (40 µM) and cobalt chloride (30 µM) influenced in vitro flowering after 25 and 45 days respectively. This is the first report on in vitro flowering in C. frutescens. The study also demonstrated successful transformation of pollen obtained from the in vitro flowers. Since capsicum is highly recalcitrant to in vitro plant regeneration, the results of the study may be highly useful in transformation of capsicum using germ free in vitro flowers.

Keywords
Capsicum frutescens, cobalt chloride, in vitro flowering, pollen transformation, regeneration, silver nitrate.

 
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