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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 11, No. 2, 2008
Bioline Code: ej08029
Full paper language: English
Document type: Note
Document available free of charge

Electronic Journal of Biotechnology, Vol. 11, No. 2, 2008

 en Universal protocol for generating 100bp size standard for endless usage
Natarajan, C.; Vijayan, N.V.; Vaidyan, L.K.; Mathew, A.; Srinivas, L. & Banerjee, M.


Developing countries are facing severe bottlenecks in the technological advancement in biotechnology, due to restrictions imposed by patent protected products and protocols. This calls for designing of simple and cost-effective alternatives for the indispensable products like DNA molecular weight markers. We demonstrate a novel, rapid and cost-effective method of making in-house 100bp ladder for routine use. In our method we use a single forward primer and five reverse primers designed on the backbone sequence of a commonly used vector template. These primers are used at a universal annealing temperature to amplify ten DNA fragments of accurate size ranging from 100bp to 1000bp. Our PCR-based method can provide size standards for an endless usage.

100bp ladder, cost effective, DNA size standards, endless usage.

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