Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
Vol. 11, No. 2, 2008
Bioline Code: ej08029
Full paper language: English
Document type: Note
Document available free of charge
Electronic Journal of Biotechnology, Vol. 11, No. 2, 2008
© Copyright 2008 - Pontificia Universidad Católica de Valparaíso -- Chile
Universal protocol for generating 100bp size standard for endless usage|
Natarajan, C.; Vijayan, N.V.; Vaidyan, L.K.; Mathew, A.; Srinivas, L. & Banerjee, M.
Developing countries are facing severe bottlenecks in the technological advancement in biotechnology, due to restrictions imposed by patent protected products and protocols. This calls for designing of simple and cost-effective alternatives for the indispensable products like DNA molecular weight markers. We demonstrate a novel, rapid and cost-effective method of making in-house 100bp ladder for routine use. In our method we use a single forward primer and five reverse primers designed on the backbone sequence of a commonly used vector template. These primers are used at a universal annealing temperature to amplify ten DNA fragments of accurate size ranging from 100bp to 1000bp. Our PCR-based method can provide size standards for an endless usage.
100bp ladder, cost effective, DNA size standards, endless usage.
Alternative site location: http://www.ejbiotechnology.info