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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 11, No. 3, 2008
Bioline Code: ej08040
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 11, No. 3, 2008

 en PCR-based sensitive detection of the edible fungus Boletus edulis check for this species in other resources from rDNA ITS sequences
Bin, L.; Jin-ping, Z.; Wei-guo, H.; Sheng, Y. & Smith, D.L.

Abstract

Identification of commercially important fungi, such as the valuable edible fungus Boletus edulis check for this species in other resources can be difficult considering visual or metabolic approaches. Based on phylogenetic analysis of the rDNA ITS sequence, a pair of specific primers was designed for differentiating B. edulis from other mushrooms by PCR. PCR was performed with total DNA as a template at an annealing temperature between 56-60ºC. Positive amplicons were obtained from B. edulis with all DNA templates from fruit bodies and cultured mycelium, but not from other fungal species at an annealing temperature of 60ºC. The result indicated that B. edulis could be clearly distinguished from other fungi by PCR, and there were no misidentifications under the reaction conditions used. The primers were also successfully employed to identify various tissues of B. edulis.

Keywords
Boletus edulis, detection, edible fungi, internal transcribed spacer, PCR, specific primers.

 
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