Apple pomace is a wasted resource produced in large
quantities and its deposit has caused serious
environmental problems, so it is significance to make
the full utilization of the residues. The objectives of this
work were to produce multienzyme bio-feed,
biodegrade the anti-nutritional factors such as pectin
and tannins in apple pomace, and obtain the nutritional
enrichment of the fermented substrate. The mixture of
apple pomace and cottonseed powder (1:1, w/w),
supplemented with 1% (w/w) (NH
4)
2SO
4 and 0.1%
(w/w) KH
2PO
4, was proved to be the optimum medium
for the mixed strains of
Aspergillus niger
M
2 and M
3
(2:1, w/w). The activities of pectinase, proteinase and
cellulase achieved 21168 u/g, 3585 u/g and 1208u/g, and
the biodegradation rates of pectin and tannins reached
99.0% and 66.1%, respectively, when 0.4%(w/w) of the
test fungiwere inoculated and incubated at 30ºC for 48
hrs in solid state fermentation. The utilization of apple pomace in the paper can be served as a model for the
similar waste recycling.
