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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 12, No. 1, 2009
Bioline Code: ej09002
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 12, No. 1, 2009

 en Bioconversion of apple pomace into a multienzyme bio-feed by two mixed strains of Aspergillus niger check for this species in other resources M2 in solid state fermentation
Zhong-Tao, S.; Lin-Mao, T.; Cheng, L. & Jin-Hua, D.

Abstract

Apple pomace is a wasted resource produced in large quantities and its deposit has caused serious environmental problems, so it is significance to make the full utilization of the residues. The objectives of this work were to produce multienzyme bio-feed, biodegrade the anti-nutritional factors such as pectin and tannins in apple pomace, and obtain the nutritional enrichment of the fermented substrate. The mixture of apple pomace and cottonseed powder (1:1, w/w), supplemented with 1% (w/w) (NH4)2SO4 and 0.1% (w/w) KH2PO4, was proved to be the optimum medium for the mixed strains of Aspergillus niger check for this species in other resources M2 and M3 (2:1, w/w). The activities of pectinase, proteinase and cellulase achieved 21168 u/g, 3585 u/g and 1208u/g, and the biodegradation rates of pectin and tannins reached 99.0% and 66.1%, respectively, when 0.4%(w/w) of the test fungiwere inoculated and incubated at 30ºC for 48 hrs in solid state fermentation. The utilization of apple pomace in the paper can be served as a model for the similar waste recycling.

Keywords
apple pomace, Aspergillus niger, multienzyme bio-feed, pectin, solid state fermentation, tannins

 
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