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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 12, No. 2, 2009
Bioline Code: ej09016
Full paper language: English
Document type: Note
Document available free of charge

Electronic Journal of Biotechnology, Vol. 12, No. 2, 2009

 en A rapid and cheap protocol for preparation of PCR templates in peanut
Wang, C.T.; Wang, X.Z.; Tang, Y.Y.; Zhang, J.C.; Yu, S.L.; Xu, Y.Z. & Bao, Z.M.


This paper describes a simple, low cost and reliable DNA template preparation protocol for polymerase chain reaction (PCR) using immature leaves from peanut seeds or leaves from field-grown plants. The technique may find wide utility in studies involving PCR-based molecular markers, rapid screening for transformants and gene cloning.

DNA extraction, groundnut, PCR, peanut

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