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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 12, No. 2, 2009
Bioline Code: ej09018
Full paper language: English
Document type: Note
Document available free of charge

Electronic Journal of Biotechnology, Vol. 12, No. 2, 2009

 en Isolation of high quality DNA: a protocol combining "rennet" and glass milk
Oliveira, L.F.V.; Wallau, G. L. & Loreto, E.L.S.

Abstract

High quality DNA is essential for many molecular biology techniques. However, the reagents used for that purpose usually are expensive and/or cause a high environmental impact. Here, we describe two alternative protocols that use inexpensive reagents and are not hazardous to the environment. The first protocol utilizes the enzyme chymosin, normally used as "rennet" in cheese production and which is easily obtained on the commercial market. The second protocol uses "rennet DNA extraction protocol" combined with the DNA binding capacity of glass powder (glass milk), which can easily be "home made". The first protocol is used when a high yield of DNA is needed, whereas the second protocol is used for production of a higher quality DNA, being able to work with sparse samples.

Keywords
chymosin, DNA extraction, glass milk, rennet, rennin, silica

 
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