Isolation of high quality DNA: a protocol combining "rennet" and glass milk|
Oliveira, L.F.V.; Wallau, G. L. & Loreto, E.L.S.
High quality DNA is essential for many molecular
biology techniques. However, the reagents used for that
purpose usually are expensive and/or cause a high
environmental impact. Here, we describe two
alternative protocols that use inexpensive reagents and
are not hazardous to the environment. The first
protocol utilizes the enzyme chymosin, normally used as
"rennet" in cheese production and which is easily
obtained on the commercial market. The second
protocol uses "rennet DNA extraction protocol"
combined with the DNA binding capacity of glass
powder (glass milk), which can easily be "home made".
The first protocol is used when a high yield of DNA is
needed, whereas the second protocol is used for
production of a higher quality DNA, being able to work
with sparse samples.
chymosin, DNA extraction, glass milk, rennet, rennin, silica