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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 12, No. 2, 2009
Bioline Code: ej09019
Full paper language: English
Document type: Note
Document available free of charge

Electronic Journal of Biotechnology, Vol. 12, No. 2, 2009

 en Measuring β-galactosidase activity at pH 6 with a differential pH sensor
Acevedo, C.; Stach, M.H.; Amtmann, A.; Young, M.E.; Reyes, J.G.; Huebner, H. & Buchholz, R.

Abstract

The β-Galactosidase activity at pH 6 is used as a cellular marker to identify senescent cell cultures. The classic method to identify this enzymatic activity is using cytochemical staining with X-Gal after 16 hrs. In this work, a differential pH sensor was used to measure β- Galactosidase activity at pH 6. The measurement is easy and only takes 3 min.

Keywords
β-Galactosidase, delta milli pH, differential pH.

 
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