en

Measuring β-galactosidase activity at pH 6 with a differential pH sensor Acevedo, C.; Stach, M.H.; Amtmann, A.; Young, M.E.; Reyes, J.G.; Huebner, H. & Buchholz, R. Abstract The β-Galactosidase activity at pH 6 is used as a cellular marker to identify senescent cell cultures. The classic method to identify this enzymatic activity is using cytochemical staining with X-Gal after 16 hrs. In this work, a differential pH sensor was used to measure β- Galactosidase activity at pH 6. The measurement is easy and only takes 3 min. Keywords β-Galactosidase, delta milli pH, differential pH.

© Copyright 2009 - Pontificia Universidad Católica de Valparaíso -- Chile
Alternative site location: http://www.ejbiotechnology.info