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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 12, No. 4, 2009
Bioline Code: ej09040
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 12, No. 4, 2009

 en Characterization of cellulolytic activities of Bjerkandera adusta check for this species in other resources and Pycnoporus sanguineus check for this species in other resources on solid wheat straw medium
Quiroz-Castañeda, R.E.; Balcázar-López, E.; Dantán-González, E.; Martinez, A.; Folch-Mallol, J. & Martínez-Anaya, C.

Abstract

Cellulolytic properties of two white rot fungi, Bjerkandera adusta check for this species in other resources and Pycnoporus sanguineus check for this species in other resources , cultivated on wheat straw agar medium, were characterized and compared. Optimal growing parameters for maximum enzyme production for both fungi were wheat straw medium pH 5 and 28ºC. B. adusta showed, on the 6th day of culture, carboxymethylcellulose (CMC)ase activity levels 1.6 times higher than maximal P. sanguineus activity, achieved on the 8th day. B. adusta supernatants also displayed higher activity levels towards xylan (3.6-fold) compared to those of P. sanguineus. However, enzymes from P. sanguineus were more robust resisting one hour incubation at high temperatures (up to 80ºC), and exhibiting activity and stability in pH range from 2 to 8. Cellulolytic activities, with molecular masses ranging from 25 to 90 kDa, from the two species were detected in zymograms.

Keywords
B. adusta, cellulases, P. sanguineus, wheat straw, xylanases.

 
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