Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
Vol. 13, No. 1, 2010
Bioline Code: ej10004
Full paper language: English
Document type: Research Article
Document available free of charge
Electronic Journal of Biotechnology, Vol. 13, No. 1, 2010
© Copyright 2010 - Pontificia Universidad Católica de Valparaíso -- Chile
Genetic diversity of breeding popcorn lines determined by SSR markers|
Trindade, A.P.R.; Pinto, R.J.B.; Amaral, A.T. Jr.; Mangolin, C.A.; da Silva Machado, M.F.P. & Scapim, C.A.
Information about genetic dissimilarity is very important to corroborate genealogical relationships and to predict the most heterozygotic hybrid combinations. Eight popcorn S6 lines of diverse germplasm types were evaluated using simple sequence repeats (SSR) markers. Of a total of 51 evaluated polymorphic primers, 15 were used for polymerase chain reaction (PCR) amplification. The genetic distance was estimated by Rogers' modified distance. The different popcorn breeding programs in Brazil are possibly using highly similar base-populations. The genetic similarity of lines P1-3 and P8-1 was lowest, while P3-3 and P8-2 were genetically more similar. The cophenetic correlation showed that the Unweighted Pair-Group Method Using Arithmetic Averages (UPGMA) was reliable to discriminate the genotypes in five groups. The clusters were consistent with the estimates of genetic identity. There was a moderate coincidence degree between the groups and genealogy of lines. Higher levels of heterozygosity are expected from crosses between the group containing lines P3-3 and P7-3 with that of P1-3 and P7-4. Crosses between lines P1-3 and P8-1 are also promising.
advanced generations, genetic variability, molecular markers, Zea mays.
Alternative site location: http://www.ejbiotechnology.info