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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 13, No. 1, 2010
Bioline Code: ej10007
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 13, No. 1, 2010

 en In vitro regeneration and genetic fidelity of Tigridia pavonia check for this species in other resources (L.f.) DC.
Piña-Escutia, J.L.; Arzate-Fernández, A.M. & Vázquez-García, L.M.

Abstract

Plants of Tigridia pavonia check for this species in other resources (L.f.) DC were regenerated from twin-scaling explants cultured on Murashige and Skoog and N6 basal medium. The highest formation of shoots per responding explant was obtained on N6 medium supplemented with 4.5 µM 2,4-dichlorophenoxy acetic acid in combination with 2.2 µM benzylaminopurine. Shoots rooted readily on N6 basal medium supplemented with 1 g l-1 activated charcoal and 2.6 µM naphtalenacetic acid. The rooted shoots achieved 100% survival. Inter Simple Sequence Repeat analysis was carried out to check for possible genetic alterations in plants obtained after two consecutive subcultures. The results revealed that the recovered plants did not exhibit any type of polymorphism.

Keywords
genetic integrity, micropropagation, oceloxóchitl

 
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