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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 13, No. 1, 2010
Bioline Code: ej10013
Full paper language: English
Document type: Short Communication
Document available free of charge

Electronic Journal of Biotechnology, Vol. 13, No. 1, 2010

 en Sequential use of ammonium and leucine as nitrogen sources during growth of Geotrichum candidum check for this species in other resources on a glucose based medium
Adour, L.; Bude, F. & Amrane, A.

Abstract

Geotrichum candidum check for this species in other resources growth on ammonium and leucine as nitrogen sources and glucose as a carbon source was examined. A clear preference of G. candidum for ammonium over leucine as a nitrogen source was shown. Indeed, ammonium was completely exhausted at the end of exponential growth after less than 35 hrs of culture; in contrast only 5% of leucine was concomitantly assimilated. Growth continued at slower rates on glucose and leucine as carbon and nitrogen sources respectively, and at the end of culture (185 hrs), leucine was completely exhausted.

Keywords
amino acids, batch culture, diauxic growth, Geotrichum candidum, growth kinetics

 
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