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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 13, No. 2, 2010
Bioline Code: ej10021
Full paper language: English
Document type: Note
Document available free of charge

Electronic Journal of Biotechnology, Vol. 13, No. 2, 2010

 en Development and application of high-throughput amplified fragment length polymorphism technique in Calluna vulgaris check for this species in other resources (Ericaceae)
Borchert, T. & Gawenda, I.

Abstract

Calluna vulgaris check for this species in other resources is an important ornamental crop of the horticultural industry in Europe. In order to improve breeding of this species, especially of the most important trait of 'bud-flowering', the implementation of molecular techniques that allow rapid, reproducible and efficient screening of whole segregating populations e.g. for molecular marker and mapping approaches is a requirement. We therefore aimed to introduce the powerful tool of amplified fragment length polymorphisms (AFLP®), a widely and successfully applied method, into our methodological assortment. As an essential prerequisite, the isolated DNA should be of adequate quality which is a common obstacle when dealing with woody species and their interfering secondary components/metabolites. The results of screening different and modified DNA isolation protocols are described. As the outcome of our evaluations of reaction conditions during the AFLP® procedure, we circumstantiate a functional protocol ranging from DNA extraction to visualization of AFLP® banding patterns for the woody crop C. vulgaris. This method is suitable for high throughput genetic applications and may even be transferable to other species. In addition, costs are reduced by reasonable reagents and multiplexing assays.

Keywords
DNA content, mapping, marker-assisted selection, ornamental, plant tissue

 
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