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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 13, No. 4, 2010
Bioline Code: ej10044
Full paper language: English
Document type: Report
Document available free of charge

Electronic Journal of Biotechnology, Vol. 13, No. 4, 2010

 en Simple method to prepare DNA templates from a slice of peanut cotyledonary tissue for Polymerase Chain Reaction
Yu, Shu Tao; Wang, Chuan Tang; Yu, Shan Lin; Wang, Xiu Zhen; Tang, Yue Yi; Chen, Dian Xu & Zhang, Jian Cheng

Abstract

An efficient DNA extraction method was developed for peanut seed, where only 3-5 mg cotyledonary tissue was enough for more than 50 PCR reactions with a reaction volume of 15 μl. Both low copy number and high copy number DNA sequences were successfully amplified. Processing one seed sample only took about half an hour. Sampling had no significant effects on germination and development. The DNA extraction method makes it possible to identify transformants and conduct molecular marker studies prior to sowing, and thus may greatly hasten research progress.

Keywords
cotyledonary tissue, DNA extraction, groundnut, PCR, peanut.

 
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