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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 13, No. 5, 2010
Bioline Code: ej10051
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 13, No. 5, 2010

 en Improved growth model for two-stage continuous cultures of Lactobacillus helveticus check for this species in other resources
Bouguettoucha, Abdallah; Balannec, Béatrice; Nacef, Saci & Amrane, Abdeltif

Abstract

An unstructured model for growth and lactic acid production during two-stage continuous cultures of Lactobacillus helveticus check for this species in other resources was previously developed. The Verlhust model was considered to describe growth kinetics. Production models was based on modified Luedeking-Piret expressions involving an inhibitory effect for the first stage (seed culture) and a nutritional limitation effect for the second stage (culture). To account for the decrease of the biomass concentration observed in the second stage, the dilution rate Dc was replaced by an exponential term of the dilution rate α exp (Dc/β ) in the growth and product relations. Contrarily to the previous model, the important decrease of the biomass concentration observed at steady state in the second stage at high dilution rates, namely close to wash out, was correctly described by the new model. It also proved to satisfactory describes production data and volumetric productivity.

Keywords
continuous culture, growth, lactic acid fermentation, substrate limitation, unstructured models.

 
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