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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 13, No. 5, 2010
Bioline Code: ej10062
Full paper language: English
Document type: Short Communication
Document available free of charge

Electronic Journal of Biotechnology, Vol. 13, No. 5, 2010

 en Novel protocol to identify true hybrids in normal oleate x high oleate crosses in peanut
Wang, Chuan Tang; Yu, Shan Lin; Zhang, Shu Wei; Wang, Xiu Zhen; Tang, Yue Yi; Zhang, Jian Cheng & Chen, Dian Xu

Abstract

A novel hybrid identification protocol was developed for F0:1 peanut seeds resulting from crosses between normal oleate cultivars with wild type FAD2B gene and high oleate genotypes with an A insertion in FAD2B gene. Presence of a series of overlapped peaks in trace file of the PCR product amplified with bF19/R1 primers was an indication of hybridity. This protocol may facilitate high oleate breeding and genetic studies in peanut.

Keywords
identification, NIRS, PCR, peanut, true hybrid.

 
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