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Novel protocol to identify true hybrids in normal oleate x high oleate crosses in peanut Wang, Chuan Tang; Yu, Shan Lin; Zhang, Shu Wei; Wang, Xiu Zhen; Tang, Yue Yi; Zhang, Jian Cheng & Chen, Dian Xu Abstract A novel hybrid identification protocol was developed for F0:1 peanut seeds resulting from crosses between normal oleate cultivars with wild type FAD2B gene and high oleate genotypes with an A insertion in FAD2B gene. Presence of a series of overlapped peaks in trace file of the PCR product amplified with bF19/R1 primers was an indication of hybridity. This protocol may facilitate high oleate breeding and genetic studies in peanut. Keywords identification, NIRS, PCR, peanut, true hybrid.

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