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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 13, No. 5, 2010
Bioline Code: ej10064
Full paper language: English
Document type: Note
Document available free of charge

Electronic Journal of Biotechnology, Vol. 13, No. 5, 2010

 en Characterization of a Gelatin/Chitosan/Hyaluronan scaffold-polymer
Enrione, Javier; Osorio, Fernando; López, Daniel; Weinstein-Oppenheimer, Caroline; Fuentes, Miguel A.; Ceriani, Ricardo; Brown, Donald I.; Albornoz, Fernando; Sánchez, Elizabeth; Villalobos, Patricio; Somoza, Rodrigo A.; Young, Manuel E. & Acevedo, Cristian A.

Abstract

Gelatin, chitosan and hyaluronic acid are natural components used to prepare polymeric scaffold in tissue engineering. The physical properties of these materials confer an appropriate microenvironment for cells, which can be used as a regeneration system for skin and cartilage. In this work, we prepared and characterized a Gelatin/Chitosan/Hyaluronan lyophilized-polymer. Physical properties of lyophilized-polymer changed slightly with moisture, but when polymer was totally hydrated the elasticity changed significantly. Thermophysical characterisation indicated that temperatures higher than 30°C could modify irreversibly the polymeric matrix probably due to protein denaturation. Besides, we used the polymer as scaffold to prepare a biosynthetic-skin, reporting biological behaviour and its mechanical properties.

Keywords
chitosan, fibrin, gelatin, hyaluronic acid, scaffold.

 
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