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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 13, No. 6, 2010
Bioline Code: ej10068
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 13, No. 6, 2010

 en Breeding of high lipid producing strain of Geotrichum robustum check for this species in other resources by ion beam implantation
Cao, Yuanyuan; Yao, Jianming; Li, Jun; Chen, Xiangsong & Wu, Jinyong


To obtain an industrial strain with high lipid yield, the wild strain G0 of Geotrichum robustum check for this species in other resources was mutated by means of nitrogen ions implantation. Mutagenic effects of strain G0 by low energy N+ ion implantation were studied. The experimental results indicated that the survival rate curve took a "saddle" shape, and the positive mutation rate was increased to 22.00% at the dose of nitrogen ions 2.0 x 1015 ions/cm2 when the survival rate was 28.60%. By repeated screening, a high lipid producing strain G9 was obtained. The biomass, lipid content and lipid yield of the mutant can reach 40.25 g/L, 71.14% and 28.63 g/L after cultured in a 5L fermenter for 8 days, increasing by 52.81%, 68.82% and 157.93% to those of wild strain, respectively. Analysis results on fatty acids composition and relative content by gas chromatography and mass spectrometry showed that the lipid in strain G9 was mainly composed of 16-carbon and 18-carbon fatty acids, including 37.360% oleic acid, 23.631% palmitic acid, 4.458% linoleic acid and 26.465% stearic acid. Such compositional features were quite similar to plant oil. Geotrichum robustum strain G9 may be an ideal high lipid producing strain for biodiesel production.

fatty acids, Geotrichum robustum, high lipid producing strain, ion beam implantation.

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