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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 13, No. 6, 2010
Bioline Code: ej10071
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 13, No. 6, 2010

 en Development of trinucleotide (GGC)n SSR markers in peanut ( Arachis hypogaea check for this species in other resources L.)
Yuan, Mei; Gong, Limin; Meng, Ronghua; Li, Shuangling; Dang, Phat; Guo, Baozhu & He, Guohao

Abstract

Cultivated peanut ( Arachis hypogaea check for this species in other resources L.) is an oilseed crop of economic importance. It is native to South America, and it is grown extensively in the semi-arid tropics of Asia, Africa, and Latin America. Given an extremely narrow genetic base, efforts are being made to develop simple sequence repeat (SSR) markers to provide useful genetic and genomic tools for the peanut research community. A SSR-enriched library to isolate trinucleotide (GGC)n SSRs in peanut was constructed. A total of 143 unique sequences containing (GGC)n repeats were identified. One hundred thirty eight primer pairs were successfully designed at the flanking regions of SSRs. A suitable polymerase was chosen to amplify these GC-rich sequences. Although a low level of polymorphism was observed in cultivated peanut by these new developed SSRs, a high level of transferability to wild species would be beneficial to increasing the number of SSRs in wild species.

Keywords
cultivated peanut, microsatellites, polymorphism, simple sequence repeat.

 
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