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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 13, No. 6, 2010
Bioline Code: ej10078
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 13, No. 6, 2010

 en Use of Candida rugosa check for this species in other resources lipase immobilized on sepabeads for the amyl caprylate synthesis: Batch and fluidized bed reactor study
Saponjic, Svetlana; Kneževic-Jugovic, Zorica D.; Bezbradica, Dejan I.; Zuza, Milena G.; Saied, Omar Ali; Boskovic-Vragolovic, Nevenka & Mijin, Dusan Z.

Abstract

Lipase from Candida rugosa check for this species in other resources was covalently immobilized on Sepabeads EC-EP for application for amyl caprylate synthesis in an organic solvent system. Several solvents were tested in terms of biocatalyst stability and the best result was obtained with isooctane. The lipase-catalyzed esterification in the selected system was performed in batch and fluidized bed reactor systems. The influence of several important reaction parameters including temperature, initial water content, enzyme loading, acid/alcohol molar ratio, and time of addition of molecular sieves is carefully analyzed by means of an experimental design. Almost complete conversion (> 99%) of the substrate to ester could be performed in a batch reactor system, using lipase loading as low as 37 mg g-1 dry support and in a relatively short time (24 hrs) at 37°C, when high initial substrate molar ratio of 2.2 is used. Kinetics in a fluidized bed reactor system seems to still have a slightly better profile than in the batch system (90.2% yields after 14 hrs). The fluidized bed reactor operated for up 70 hrs almost with no loss in productivity, implying that the proposed process and the immobilized system could provide a promising approach for the amyl caprylate synthesis at the industrial scale.

Keywords
bioreactors, covalent immobilization, ester, non-aqueous system, optimization technique.

 
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