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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 13, No. 6, 2010
Bioline Code: ej10079
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 13, No. 6, 2010

 en Callus induction and plant regeneration of the endemic Astragalus nezaketae check for this species in other resources in Turkey
Erisen, Semiha; Yorgancilar, Mustafa; Atalay, Emine; Babaoglu, Mehmet & Duran, Ahmet

Abstract

A callus induction and plant regeneration protocol was developed from leaf and petiole explants of the endemic Astragalus nezaketae check for this species in other resources . Explants were cultured on Murashige and Skoog medium (MS) supplemented with different plant growth regulators (PGRs) [a-naphthaleneacetic acid (NAA), benzyladenine (BA), 2,4-dichlorophenoxyacetic acid (2,4-D), kinetin (Kin), thidiazuron (TDZ)]. The combinations and concentrations of PGRs were shown significant variations for the frequency of callus formation, appearence of callus and the potential of callus differentiation. NAA x BA have been found highly affective in callusing and plant regeneration. Other PGRs have not resulted in callus differentiation for shoot formation. The highest number of shoots (6/explants) was obtained from leaf explants cultured on MS with 0.5 mg/l NAA and 4 mg/l BA. The regenerated shoots transferred to rooting medium (MS with 0.5 mg/l indole-3-butyric acid) were successfully rooted (100%) and showed rapid elongation. Rooted plantlets were acclimatized in pots containing 1:1 mixture of peat and perlite.

Keywords
astragalus, callus, organogenesis, plant regeneration.

 
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