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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 14, No. 1, 2011
Bioline Code: ej11002
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 14, No. 1, 2011

 en Assessment of genetic diversity and relationships among Osmanthus fragrans check for this species in other resources cultivars using AFLP markers
Wang, Jun Yuan; Yuan, Ji Han; Mei, Fang Dong & Fu, De Shang


This study was conducted to reveal genetic diversity among 100 Osmanthus fragrans check for this species in other resources cultivars using amplified fragment length polymorphism (AFLP) markers. Eight AFLP primer combinations produced a total of 443 polymorphic fragments with an average of 64 per primer combination. The percentage of polymorphic bands (86.81%), the resolving power (Rp) (32.71) and the PIC values (0.331) showed the efficiency of used primer combinations. The revealed AFLP makers were effective in distinguishing all the cultivars considered. Cluster analysis were performed to assess patterns of diversity among cultivars and showed the abundant genetic diversity. The overall distribution pattern of molecular variation suggested that 93.33% of the total genetic variance was within the identified groups and 6.67% of the genetic variation was among the identified groups. Our results showed that AFLP markers are useful for Osmanthus fragrans germplasm discrimination as well as for investigation of genetic diversity and variation. The information will facilitate germplasm identification, conservation and new cultivar development

AFLP marker, cluster analysis, genetic diversity, Osmanthus fragrans.

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