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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 14, No. 1, 2011
Bioline Code: ej11004
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 14, No. 1, 2011

 en Ethanol production from sweet sorghum juice under very high gravity conditions: Batch, repeated-batch and scale up fermentation
Sunan Nuanpeng; Lakkana Laopaiboon; Penjit Srinophakun; Preekamol Klanrit; Prasit Jaisil & Pattana Laopaiboon

Abstract

Batch ethanol fermentations from sweet sorghum juice by Saccharomyces cerevisiae check for this species in other resources NP 01 were carried out in a 500 ml air-locked Erlenmeyer flask under very high gravity (VHG) and static conditions. The maximum ethanol production efficiency was obtained when 9 g l-1 of yeast extract was supplemented to the juice. The ethanol concentration (P), productivity (Qp) and yield (Yp/s) were 120.24 ± 1.35 g l-1, 3.01 ± 0.08 g l-1 h-1 and 0.49 ± 0.01, respectively. Scale up ethanol fermentation in a 5-litre bioreactor at an agitation rate of 100 rev min-1 revealed that P, Qp and Yp/s were 139.51 ± 0.11 g l-1, 3.49 ± 0.00 g l-1 h-1 and 0.49 ± 0.01, respectively, whereas lower P (119.53 ± 0.20 g l-1) and Qp (2.13 ± 0.01 g l-1 h-1) were obtained in a 50-litre bioreactor. In the repeated-batch fermentation in the 5-litre bioreactor with fill and drain volume of 50% of the working volume, lower P and Qp were observed in the subsequent batches. P in batch 2 to 8 ranged from 103.37 ± 0.28 to 109.53 ± 1.06 g l-1.

Keywords
batch, ethanol fermentation, repeated-batch, scale up, sweet sorghum, VHG.

 
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