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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 14, No. 1, 2011
Bioline Code: ej11010
Full paper language: English
Document type: Note
Document available free of charge

Electronic Journal of Biotechnology, Vol. 14, No. 1, 2011

 en A simple ethanol wash of the tissue homogenates recovers high-quality genomic DNA from Corchorus check for this species in other resources species characterized by highly acidic and proteinaceous mucilages
Kundu, Avijit; Sarkar, Debabrata; Bhattacharjee, Amit; Topdar, Niladri; Sinha, Mohit Kumar & Mahapatra, Bikash Sinha

Abstract

A simple miniprep based on early elimination of highly acidic and proteinaceous mucilages through ethanol washing of the tissue homogenates has been developed for the extraction of genomic DNA from mature leaves and seeds of Corchorus check for this species in other resources spp. As compared to high cetyltrimethylammonium bromide (CTAB)-NaCl DNA extraction followed by ethanol-based removal of remnant mucilages from the DNA pellet, this simple miniprep consistently and reproducibly recovers high amounts of DNA with good spectral qualities at A260/A280 and A260/A230. The purified DNA is efficiently digested by restriction endonucleases, and is suitable for PCR amplification of nuclear microsatellites with expected allele sizes.

Keywords
Corchorus, DNA extraction, microsatellite, mucilage, PCR, restriction digestion.

 
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