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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 14, No. 2, 2011
Bioline Code: ej11016
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 14, No. 2, 2011

 en Characterization of novel genic SSR markers in Linum usitatissimum (L.) and their transferability across eleven Linum species
Soto-Cerda, Braulio J.; Saavedra, Hector Urbina; Navarro, Cristell Navarro & Ortega, Paula Mora

Abstract

Little is known about the evolutionary relationships among Linum species, basically because of the lack of transferable molecular markers. Currently, expressed sequence tags available in public databases provide an opportunity for the rapid and inexpensive development of simple sequence repeat (SSR) markers in wild flax species. In this regard, fifty expressed sequence tag-derived microsatellite markers (EST-SSRs) were evaluated for polymorphism and transferability in 50 Linum usitatissimum cultivars/accessions and 11 Linum species. Among them 23 EST-SSRs were polymorphic in L. usitatissimum, while 2-4 alleles were detected (average 2.26 per locus). The polymorphism information content value ranged from 0.08 to 0.55 (average 0.38). Forty one genic markers (95.3%) produced strong amplicons in at least two of the 11 Linum species. The percentage of cross amplification ranged from 34.1% to 92.7% in L. tauricum and L. bienne, respectively. Moreover, the rate of transferability was associated positively with the botanical section. Our results suggest that the high degree of EST-SSRs transferability to Linum species can be a useful enhancement of the current database of SSR markers for future genetic and evolutionary studies.

Keywords
EST-SSRs, expressed sequence tag, flaxseed, polymorphic loci, wild relatives.

 
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