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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 14, No. 2, 2011
Bioline Code: ej11022
Full paper language: English
Document type: Short Communication
Document available free of charge

Electronic Journal of Biotechnology, Vol. 14, No. 2, 2011

 en Sodium azide mutagenesis resulted in a peanut plant with elevated oleate content
Wang, Chuan Tang; Tang, Yue Yi; Wang, Xiu Zhen; Zhang, Shu Wei; Li, Gui Jie; Zhang, Jian Cheng & Yu, Shan Lin

Abstract

Screening of peanut seeds resulting from 0.39% sodium azide treatment with NIRS calibration equation for bulk seed samples identified a plant with more than 60% oleate. Oleate content in individual seeds of the plant, as predicted by NIRS calibration equation for intact single peanut seeds, ranged from 50.05% ~ 68.69%. Three seeds with >60% oleate thus identified were further confirmed by gas chromatography. Multiple sequence alignments of the FAD2B gene from Huayu 22 (wild type) and peanut seeds with elevated oleate (mutant type) revealed a C281T transition in the coding region causing an I94T substitution in the oleoyl-PC desaturase, which may be responsible for reduction in the enzyme activity.

Keywords
chemical mutant, FAD2B, NIRS, oleate, peanut.

 
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