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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 14, No. 3, 2011
Bioline Code: ej11035
Full paper language: English
Document type: Review Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 14, No. 3, 2011

 en A preferable approach to clone hLIF cDNA from the genomic DNA
Cui, Yihong; Zhu, Guangqin; Chen, Qiuju; Wang, Yunfei; Yang, Minming; Song, Yuxuan; Wang, Jiangang & Cao, Binyun

Abstract

Complementary DNA (cDNA) is valuable for investigating protein structure and function in the research of life science, but it is difficult to obtain by traditional reverse transcription. In this study, we employed a novel strategy to clone the human leukemia inhibitory factor (hLIF) gene cDNA from genomic DNA directly isolated from the mucous membrane of mouth. The hLIF sequence can be acquired within a few hours by means of amplification of each exon and splicing using overlap-PCR. Thus, the new approach developed in this study is simple, time- and cost-effective, and it is not limited to particular gene expression levels of each tissue.

Keywords
cDNA cloning, hLIF, oral mucous membrane, overlap PCR

 
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