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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 14, No. 5, 2011
Bioline Code: ej11050
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 14, No. 5, 2011

 en Antimicrobial effects of plant defence peptides expressed by bovine endothelial cells on intracellular pathogens
Loeza-Ángeles, Heber; López-Meza, Joel E. & Ochoa-Zarzosa, Alejandra


Background: The actions of plant antimicrobial peptides (PAP) against intracellular pathogens are poorly known. It has been reported that wheat puroindolines show antibacterial activity against Staphylococcus epidermidis check for this species in other resources endocyted by macrophages. In this work, we evaluated the intracellular antimicrobial activity of PAP γ-thionin and thionin Thi2.1 produced by bovine endothelial cells against intracellular Staphylococcus aureus check for this species in other resources and Candida albicans check for this species in other resources . We used three host-pathogen models: 1) bovine mammary epithelial cells (BMEC)-S. aureus, 2) bovine endothelial cells (BEC)-S. aureus and 3) BEC-C. albicans, and evaluated the effect of conditioned media from BEC producers of PAP (γ-thionin and thionin Thi2.1).
Results: In the first model, conditioned medium (CM) containing Thi2.1 completely inhibited S. aureus intracellular after 24 hrs treatment. In the second model, CM from BEC containing γ-thionin has a better effect killing intracellular S. aureus for 12-24 hrs incubations than CM from endothelial cells producers of Thi2.1; this was related with an increase of nitric oxide production (~2 times) in BEC infected and treated for 12 hrs with CM containing γ-thionin, which negatively correlates with bacterial viability. In the third model, CM containing Thi2.1 showed a more potent intracellular fungicidal activity (~85% of inhibition) at 24 hrs treatment than CM containing γ-thionin (~35% of inhibition).
Conclusions: This work shows new effects of PAP to control intracellular bacterial or fungal infections.

antimicrobial peptides, Arabidopsis thaliana thionin, Capsicum chinense γ-thionin, Candida albicans, intracellular pathogens, Staphylococcus aureus

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