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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 14, No. 5, 2011
Bioline Code: ej11054
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 14, No. 5, 2011

 en Identification of chilling-responsive transcripts in peanut ( Arachis hypogaea check for this species in other resources L.)
Tang, Yue Yi; Wang, Chuan Tang; Yang, Guan Pin; Feng, Tong; Gao, Hua Yuan; Wang, Xiu Zhen; Chi, Xiao Yuan; Xu, Ya Long; Wu, Qi & Chen, Dian Xu

Abstract

To isolate differentially expressed peanut genes responsive to chilling, a suppression subtractive hybridization (SSH) cDNA library was constructed for a chilling tolerant peanut cultivar A4 with mRNAs extracted from the seeds imbibed at 2°C and 15°C, respectively, for 24 hrs. A total of 466 cDNA clones were sequenced, from which 193 unique transcripts (73 contigs and 120 singlets) were assembled. Of these unique transcripts, 132 (68.4%) were significantly similar to the sequences in GenBank non-redundant (nr) protein database, which belonged to diverse functional categories including metabolism, signal transduction, stress response, cell defense and transcriptional regulation. The remaining 61 (31.6%) showed no similarity to either hypothetical or known proteins. Six differentially expressed transcripts were further confirmed with real-time quantitative PCR (RT-qPCR).

Keywords
chilling response, peanut, real-time quantitative PCR, suppression subtractive hybridization

 
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