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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 14, No. 6, 2011
Bioline Code: ej11066
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 14, No. 6, 2011

 en Cloning and analysis of a NBS-LRR disease resistance gene candidate PnAG1 from peanut ( Arachis hypogaea check for this species in other resources L.)
Shan, Shi-hua; Zhang, Ting-ting; Li, Chun-juan; Yang, Chen; Yan, Cai-xia & Wan, Shu-bo

Abstract

Background: Based on the conserved sequences of a known NBS resistance gene, a pair of degenerate primers was designed to amplify the NBS-LRR resistance gene from peanut using PCR and RACE methods.
Results: Analyzing the amino acid sequence by BLAST on NCBI, which was deduced from the 1088bp-long gene named PnAG1-2, showed that it had a certain homology with some resistance proteins, among which Arachis cardenasii check for this species in other resources resistance protein gene had the highest homology (66%). Relative quantification PCR analysis indicated that PnAG1-2 gene expresses more in J11 (an A. flavus-resistant variety) than in JH1012 (an A. flavus-susceptible variety) when the harvest time was coming.
Conclusions: In this study, the NBS-LRR resistance sequence was successfully cloned from peanut and prokaryotic expression was done on the gene, which provided a foundation for cultivating anti-A. flavus peanut varieties.

Keywords
bioinformatics, NBS-LRR, peanut, real-time fluorescence quantitative PCR

 
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