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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 15, No. 1, 2012
Bioline Code: ej12003
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 15, No. 1, 2012

 en Biodegradation kinetics of o-cresol by Pseudomonas putida check for this species in other resources DSM 548 (pJP4) and o-cresol removal in a batch-recirculation bioreactor system
Pazarlioglu, Nurdan Kasikara; Kaymaz, Yasin & Babaoğlu, Anıl

Abstract

The biodegradation kinetics of o-cresol was examined by acclimatized P. putida check for this species in other resources DSM 548 (pJP4) in batch experiments at varying initial o-cresol concentrations (from 50 to 500 mg/L). The kinetic parameters of o-cresol aerobic biodegradation were estimated by using the Haldane substrate inhibition equation. The biodegradation kinetics of o-cresol was investigated. In batch culture reactors, the Maximum specific growth rate (μmax), Monod constant (Ks) and the inhibition constant (Ki) were established as 0.519 h-1, 223.84 mg/L and 130.883 mg/L, respectively. o-cresol biodegradation in a batch-recirculation bioreactor system by immobilized P. putida was also studied. The recycled packed bed reactor system, which was composed of Ca-alginate beads and pumice on which cells immobilized, has been performed to determine possible stability for further developments.

Keywords
biodegradation, cell immobilization, o-cresol, Pseudomonas putida, substrate inhibition kinetics

 
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