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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 15, No. 2, 2012, pp. 1-8
Bioline Code: ej12014
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 15, No. 2, 2012, pp. 1-8

 en Expression of RNA polymerase IV and V in Oryza sativa check for this species in other resources
de Rocio Canche Moo, Leydi; González, Angela Kú; Rodríguez-Zapata, Luis Carlos; Suarez, Victor & Castaño, Enrique

Abstract

Abstract RNA polymerase IV and V are principal players in the RdDM pathway, where their current study has shown interaction of several factors that control DNA silencing of intergenic regions and siRNA production. DNA silencing is an important process during cell differentiation, nuclear structure and viral control. However, RNA pol IV and V are yet to be study in model monocot systems like Oryza sativa check for this species in other resources that can provide further information on genetic silence mechanism in plats. We show the expression pattern of these polymerases in tissues extracts of Oryza sativa. Detectable amounts of these polymerases are found in specific adult plant tissues and particularly expressed during somatic embryogenesis but not during early stages of normal embryo development. The use of synthetic auxin leads to an induction of both RNA pol IV and V in scutellum tissue where nuclear localization may be required for genome reorganization and gene silencing.

Keywords
Oryza sativa; RNA pol IV; RNA pol V; somatic embryogenesis; zygotic embryogenesis

 
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