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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 15, No. 3, 2012, pp. 1-7
Bioline Code: ej12022
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 15, No. 3, 2012, pp. 1-7

 en Characterization of extracellular cellulose-degrading enzymes from Bacillus thuringiensis check for this species in other resources strains
Lin, Ling; Kan, Xianzhao; Yan, Hao & Wang, Danni

Abstract

The gram-positive spore-forming bacteria, Bacillus thuringiensis check for this species in other resources (Bt) strains produced novel cellulases which could liberate glucose from soluble cellulose, carboxymethyl cellulose (CMC), and insoluble crystalline cellulose. The maximal cellulase activities were obtained after 60 hrs incubation at 28ºC in a LB broth medium with 1% CMC. Maximum CMCase activities were got at 40ºC and pH 4.0, respectively, and more than 50% of its maximal activity was retained at 40-60ºC for 1 hr, while approximately 40% of its maximal activity was also retained after incubating at 70ºC for 1 hr. Most metal ions and reagents such as Ca2+, Mg2+, Cd2+, Pb2+, Zn2+, Cu2+, EDTA, and SDS inhibited the enzyme activities, but K+ and Mn2+ activated the activities. The enzymes from Bacillus thuringiensis strains could be applied in bioconversion of lignocellulosic biomass into fermentable sugars.

Keywords
Bacillus thuringiensis; cellulase; cellulose-degrading; characterization

 
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