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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 15, No. 3, 2012, pp. 1-8
Bioline Code: ej12024
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 15, No. 3, 2012, pp. 1-8

 en Effects of fermentation substrates and conservation methods on the viability and antimicrobial activity of Weissella confusa check for this species in other resources and its metabolites
Serna-Cock, Liliana; Enríquez-Valencia, Cruz E.; Jiménez-Obando, Eliana M. & Campos-Gaona, Rómulo

Abstract

Abstract Lactic acid bacteria produce metabolites with antagonistic activity against other bacteria. However, growth conditions and conservation methods may reduce the viability and antimicrobial activity of lactic acid bacteria. This study evaluated the effects of fermentation substrate, lyophilization (freeze-drying) and refrigeration on the viability and antimicrobial activity of Weissella confusa check for this species in other resources strain and its metabolites against pathogens responsible for bovine mastitis. W. confusa strain was grown in MRS broth and milk supplemented with yeast extract and glucose (MYEG). The collected fractions were preserved by lyophilization or under refrigeration at 4ºC. Every seven days, the viability of W. confusa strain and the stability of its metabolites were evaluated against Staphylococcus aureus check for this species in other resources and Streptococcus agalactiae check for this species in other resources by disc diffusion assays. In both fermentation substrates, the combination of lyophilized strain and metabolites retained antimicrobial activity against the two pathogens for 42 days. Also, W. confusa strain retained adequate viability and antimicrobial activity when grown in MYEG and stored under refrigeration conditions. It was concluded that MYEG and refrigeration are acceptable low cost options to preserve the viability of W. confusa for its potential commercial use in the prevention and treatment of bovine mastitis.

Keywords
conservation; inhibition; lactic acid bacteria; lyophilization

 
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